2014年11月27日公開iPS細胞関連特許
2014/11/28
Pub. No.: WO/2014/190361 International Application No.: PCT/US2014/040362
Publication Date: 27.11.2014 International Filing Date: 30.05.2014
IPC:
Applicants: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS, INC. [US/US]; 6404 Nancy Ridge Drive San Diego, California 92121-2248 (US)
Inventors: WANG, Jiwu; (US)
Agent: NORTON, Vicki G.; (US)
Priority Data:
61/646,292 13.05.2012 US (Priority Withdrawn 02.10.2014)
13/893,166 13.05.2013 US
Title (EN) FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA
(EN)The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.
Pub. No.: WO/2014/189144 International Application No.: PCT/JP2014/063753
Publication Date: 27.11.2014 International Filing Date: 23.05.2014
IPC:
C12Q 1/02 (2006.01), A61K 35/12 (2006.01), A61K 35/14 (2006.01), A61P 35/02 (2006.01), C12N 5/10 (2006.01)
Applicants: KYOTO UNIVERSITY [JP/JP]; 36-1, Yoshida-honmachi, Sakyo-ku, Kyoto-shi, Kyoto 6068501 (JP)
Inventors: YAMANAKA, Shinya; (JP).
YOSHIDA, Yoshinori; (JP).
CHONABAYASHI, Kazuhisa; (JP)
Agent: TAKASHIMA, Hajime; Meiji Yasuda Seimei Osaka Midosuji Bldg., 1-1, Fushimimachi 4-chome, Chuo-ku, Osaka-shi, Osaka 5410044 (JP)
Priority Data:
2013-109385 23.05.2013 JP
(JA) 骨髄異形成症候群等の治療／予防薬のスクリーニング方法
Abstract: front page image
(JA) 本発明は、急性骨髄性白血病または骨髄異形成症候群の治療または予防薬をスクリーニングする方法であって、以下の工程： （ａ）骨髄異形成症候群患者から単離した血液単核球中の非T細胞から製造した人工多能性幹（iPS）細胞から誘導された造血前駆細胞を被検物質の存在下および非存在下で、コロニーを形成させる工程、および （ｂ）被検物質の存在下でのコロニー数が、被検物質の非存在下でのコロニー数と比較して、増加した場合、当該被検物質を急性骨髄性白血病または骨髄異形成症候群の治療または予防薬の候補として選出する工程、 を含む、方法等を提供する。
Publication Date: 27.11.2014 International Filing Date: 30.05.2014
IPC:
Applicants: ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS, INC. [US/US]; 6404 Nancy Ridge Drive San Diego, California 92121-2248 (US)
Inventors: WANG, Jiwu; (US)
Agent: NORTON, Vicki G.; (US)
Priority Data:
61/646,292 13.05.2012 US (Priority Withdrawn 02.10.2014)
13/893,166 13.05.2013 US
Title (EN) FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA
(EN)The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.
Pub. No.: WO/2014/189144 International Application No.: PCT/JP2014/063753
Publication Date: 27.11.2014 International Filing Date: 23.05.2014
IPC:
C12Q 1/02 (2006.01), A61K 35/12 (2006.01), A61K 35/14 (2006.01), A61P 35/02 (2006.01), C12N 5/10 (2006.01)
Applicants: KYOTO UNIVERSITY [JP/JP]; 36-1, Yoshida-honmachi, Sakyo-ku, Kyoto-shi, Kyoto 6068501 (JP)
Inventors: YAMANAKA, Shinya; (JP).
YOSHIDA, Yoshinori; (JP).
CHONABAYASHI, Kazuhisa; (JP)
Agent: TAKASHIMA, Hajime; Meiji Yasuda Seimei Osaka Midosuji Bldg., 1-1, Fushimimachi 4-chome, Chuo-ku, Osaka-shi, Osaka 5410044 (JP)
Priority Data:
2013-109385 23.05.2013 JP
(JA) 骨髄異形成症候群等の治療／予防薬のスクリーニング方法
Abstract: front page image
(JA) 本発明は、急性骨髄性白血病または骨髄異形成症候群の治療または予防薬をスクリーニングする方法であって、以下の工程： （ａ）骨髄異形成症候群患者から単離した血液単核球中の非T細胞から製造した人工多能性幹（iPS）細胞から誘導された造血前駆細胞を被検物質の存在下および非存在下で、コロニーを形成させる工程、および （ｂ）被検物質の存在下でのコロニー数が、被検物質の非存在下でのコロニー数と比較して、増加した場合、当該被検物質を急性骨髄性白血病または骨髄異形成症候群の治療または予防薬の候補として選出する工程、 を含む、方法等を提供する。
